2015年生物科学论坛第三十二期

时间�?015�?0�?9日上�?0: 00
地点：生物楼313
报告题目：�?D� Analysis Pipeline: Investigate Diversity/Dynamics and Perform Differential Testing of Next-Generation Sequencing Based T cell Repertoire Data
报告人：Li Zhang, Ph.D.（University of California, San Francisco�
报告人介绍：
Li Zhang,Associate Adjunct ProfessorofUniversity of California, San Francisco. She received her PhD degrees in Statistics from the University of Florida. She focuses on Case-Control Study, Cancer Epidemiology, Cancer Genetics/Genomics, Clinical Trial for Cancer Research, Genetic Epidemiology,Statistics Method development, TCR sequencing.
报告内容�
Cancer immunotherapy has demonstrated significant clinical activity in many cancers. The mechanism by which these treatments work in patients is not fully elucidated. Nevertheless, T-cells represent a crucial component of the adaptive immune system that is thought to play a significant role in anti-tumoral immunity. The part of the T-cell responsible for antigen-specific recognition is the T-cell receptor (TCR). The diversity and dynamics of T-cells that exist in an individual’s TCR repertoire remains unknown. Next generation sequencing (NGS) of the TCRs can be used as a platform to quantitate T cell clonotypes. However, currently there is no established analysis pipeline to track and examine the dynamic nature of the TCR repertoire.
The �?D� analysis pipeline is the advanced analysis of TCR repertoires after primary sequences extraction from raw sequencing reads. It integrates not only the various repertoire diversity measures and commonly used approaches to the repertoire analysis but also our newly proposed PDIS approach and modified DEseq method for high-throughput sequencing data. The pipeline has proven its utility in recent research studies. We believe that the establishment of this analysis pipeline will provide researchers with a guideline and parameters to consider for future studies using NGS-based TCR repertoire profiling.

本次论坛联系人：邬荣领教掇�/P>

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